Gélose m-Endo, LES

Taille

500g

500 g (en gros)

2 kg

2 kg (vente en gros)

10 kg

10 kg (vente en gros)

Prix

Quantité

Paiements sécurisés

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Gélose m-Endo, LES

La gélose m Endo, LES, est utilisée pour le dénombrement des coliformes dans l'eau par filtration membranaire. La digestion enzymatique de caséine et d'extrait de levure apporte des composés azotés et des vitamines, le lactose étant le glucide fermentescible. L'association de sulfite de sodium, de désoxycholate de sodium et de fuchsine basique inhibe la croissance de nombreux micro-organismes Gram positifs et Gram négatifs. Le laurylsulfate de sodium agit comme agent sélectif contre les organismes non coliformes. Les coliformes fermentant le lactose produisent des colonies rose-rouge avec un reflet vert caractéristique, tandis que les bactéries incapables de fermenter ce sucre forment des colonies rose pâle ou incolores. Le chlorure de sodium est utilisé pour maintenir l'équilibre osmotique du milieu, et les phosphates de potassium agissent comme agents tampons. L'agar est l'agent gélifiant. Trois formats sont disponibles : 500 g, 2 kg et 10 kg.

PRÉPARATION : Mélanger 51 grammes de milieu dans 980 ml d’eau purifiée et 20 ml d’éthanol à 95 % jusqu’à dispersion homogène. Chauffer en remuant plusieurs fois jusqu’à dissolution complète. NE PAS AUTOCLAVER.

Littérature supplémentaire :

Informations sur le produit M13-124

Fiche de données de sécurité M13-124

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