Gélose d'isolement de Pseudomonas

Taille

500g

500 g (en gros)

2 kg

2 kg (vente en gros)

10 kg

10 kg (vente en gros)

Prix

Quantité

Paiements sécurisés

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Gélose d'isolement de Pseudomonas

La gélose d'isolement de Pseudomonas est utilisée pour l'isolement sélectif de Pseudomonas et la différenciation de Pseudomonas aeruginosa par la production de pigments. La digestion enzymatique de gélatine fournit des sources d'azote pour la croissance bactérienne. La présence d'irgasan permet la croissance de Pseudomonas spp . tout en inhibant celle des micro-organismes Gram-positifs et de certains Gram-négatifs. Le chlorure de magnésium et le sulfate de potassium stimulent la production de chélateurs du fer, la pyoverdine (fluorescente jaune-vert ou jaune-brun) et la pyocianine (bleue), dont la combinaison produit une couleur vert vif caractéristique chez P. aeruginosa . L'agar est l'agent gélifiant. Trois formats sont disponibles : 500 g, 2 kg et 10 kg.

PRÉPARATION : Mélanger 45 g de milieu gélosé dans 980 ml d’eau purifiée. Ajouter 20 ml de glycérol tout en chauffant pour faciliter l’écoulement de la pipette. Chauffer en remuant plusieurs fois jusqu’à ébullition pour dissoudre complètement. Répartir et autoclaver à 121,0 °C pendant 15 minutes.

Littérature supplémentaire :

Informations sur le produit P16-114

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