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XLD Agar is used for isolating and differentiation of Gram-negative enteric bacteria. In this medium, Yeast Extract provides the nitrogenous sources and carbohydrates required for bacterial growth. Sodium Chloride supplies electrolytes and maintains osmotic equilibrium. Xylose, Lactose and Sucrose are the fermentable carbohydrates, being the former utilized by most enteric bacteria except for Shigella and Providencia spp. Upon Xylose depletion, Salmonella (but not non-pathogen Xylose fermenters) decarboxylates L-Lysine and reverts the pH acidification due to carbohydrate fermentation. Phenol Red serves as pH indicator. Sodium Thiosulfate and Ferric Ammonium Citrate detect hydrogen sulfide formation in alkaline conditions. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 55 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil until dissolved completely. DO NOT AUTOCLAVE.