SIM Medium - Sulfide Indole Motility Medium

SIM Medium, also known as Sulfide Indole Motility Medium, is a semisolid dehydrated culture medium used by trained laboratory personnel for the determination of sulfide production, indole formation, and motility in Enterobacteriaceae testing workflows.

This medium is commonly used in microbiology, food testing, water and environmental testing, quality control, teaching, research, and industrial laboratory settings where laboratories require a prepared medium for differential biochemical observation.

Why Choose SIM Medium?

• Three-in-one differential medium: Supports sulfide, indole, and motility observations in one semisolid medium.
• Semisolid formulation: The low agar concentration allows motility to be observed by growth movement away from the original inoculation line.
• Hydrogen sulfide detection: The formulation includes ferrous ammonium citrate and sodium thiosulfate for sulfide production observation.
• Indole detection workflow: Casein peptone supplies tryptophan, supporting indole formation observation when used with the appropriate reagent and approved lab procedure.
• Multiple package sizes: Available in 500 g, 2 kg, and 10 kg sizes to match routine lab, institutional, and higher-volume usage.
• Canadian purchasing support: Supplied by Cambridge Environmental Products with CAD pricing and support for Canadian laboratory buyers.

Available Sizes

SIM Medium Product Details

Technical Notes for Laboratory Buyers

SIM Medium is a semisolid medium. Motility can be observed by growth movement away from the original stab line, while non-motile organisms typically remain closer to the inoculation line. Sulfide production is indicated by the formation of a black precipitate, and indole observation is performed using the appropriate reagent according to the laboratory’s approved procedure.

This product is intended for use by trained laboratory personnel following approved microbiology procedures, facility safety requirements, and applicable quality control practices. Always refer to the manufacturer literature, internal SOPs, and required safety documentation before use.

Common Questions About SIM Medium

What is SIM Medium used for?

SIM Medium is used in microbiology laboratories for sulfide production, indole formation, and motility observations in Enterobacteriaceae testing workflows.

What does SIM stand for?

SIM stands for Sulfide, Indole, and Motility. The medium supports observation of these three characteristics in one semisolid medium.

What package sizes are available?

SIM Medium is available in 500 g, 2 kg, and 10 kg package sizes.

What is the final pH of SIM Medium?

The final pH is listed as 7.3 ± 0.2 at 25°C.

How should SIM Medium be stored?

Store the sealed container at 2 to 30°C. Once opened and recapped, keep the container in a low-humidity environment at the same storage temperature and protect it from moisture and light.

Where can I buy SIM Medium in Canada?

Cambridge Environmental Products supplies SIM Medium in Canada with CAD pricing, Canadian purchasing support, and package sizes for routine microbiology and QC laboratory workflows.

Is SIM Medium suitable for teaching or quality control labs?

Yes. SIM Medium is commonly used in teaching, research, food, environmental, water, and quality control microbiology workflows where approved laboratory procedures are followed.

Additional Literature:
S19-110 SIM Medium Product Information