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R2A Agar medium is used for the detection of bacteria in water, especially in treated potable water. This is a low-nutrient medium in which a combination of longer time and lower temperature of incubation promotes the growth of stressed and chlorine-treated bacteria. Enzymatic Digest of Casein and Animal Tissue provide nitrogenous sources for bacterial growth. Yeast Extract supplies B-complex vitamins and serves as growth enhancer. Dextrose is the fermentable carbohydrate. Soluble starch serves as neutralizer, while Sodium Pyruvate stimulates the growth of stressed bacteria. Dipotassium Phosphate and Magnesium Sulfate Heptahydrate act as pH buffers. Agar is the gelifying agent. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 18.2 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Autoclave at 121°C for 15 minutes.