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Palcam Agar is used for the selective isolation and enumeration of Listeria monocytogenes from foods and environmental samples. Columbia Blood Agar Base is a general-purpose growth medium. Mannitol and Phenol Red have been added to differentiate Mannitol-fermenting strains of possible contaminants. Polymyixin B, Acriflavin, Ceftazidime, and Lithium Chloride are selective agents used to suppress the growth of Gram-negative and certain Gram-positive bacteria. Esculin and Dextrose are the main energy sources for bacterial growth. Members of the Listeria group hydrolyze Esculin to 6,7-dihydroxycoumarine, which reacts with Ferric Ammonium Citrate and results in the blackening of the medium. Agar is the gelifying agent. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 74 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add sterile solution of Polymyixin B 10mg/L, Acriflavin 5.0mg/L and Ceftazidime, 20mg/L.