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Mannitol Salt Agar
Mannitol Salt Agar is a medium used for isolating and differentiating both pathogenic and non-pathogenic Staphylococci. The high concentration of Sodium Chloride makes this medium selective for Gram-positive bacteria. D-Mannitol is the carbohydrate source, while Beef Extract and the enzymatic digest of Casein and Animal Tissue supply amino acids, peptides, and other nitrogenous sources. On this medium, Staphylococcus aureus strains produce large yellow colonies surrounded by yellow zones while non-pathogenic, mannitol-fermenting Staphylococci form smaller, red colonies due to the color shift of the pH indicator Phenol Red. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 111 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Autoclave at 121°C for 15 minutes.