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Lactose Broth is a liquid medium used for the detection and/or verification of the presence of Salmonella and other coliform organisms in water, foods, dairy, and pharmaceutical products. This medium is also used as a pre-enrichment in the analysis of food, milk products, and water for Salmonella. Lactose Broth also provides good growth characteristics for coliforms and fermentation is observed by gas bubbles present in an inverted fermentation tube. The concentration of media prepared for the test should be adjusted to accommodate a volume of sample greater than 10mL. Pancreatic Digest of Gelatin and Beef Extract provide nitrogenous, carbon and vitamin sources, being Lactose the fermentable carbohydrate. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 13 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.