Hygiena InSite Listeria is an all-in-one swab test for screening food processing environments for Listeria species. Swab the surface, snap to release the selective broth, and incubate at 37 °C. If Listeria is present, the yellow medium turns grey or black, with presumptive positives visible from 24 hours and negatives confirmed at 48 hours. There's no media prep, no plating and no lab equipment beyond a 37 °C incubator.
Hygiena states InSite Listeria detects fewer than 10 CFU of Listeria spp. and holds AOAC Research Institute Performance Tested Methods℠ certification. It's sold in boxes of 50 tests. Hygiena InSite Listeria is available in Canada from Cambridge Environmental Products with CAD pricing and Canadian purchasing support.
The pre-moistened foam swab, selective broth and colour indicator are in one sealed device, so there's no media to prepare or transfer and less risk of cross-contamination.
Listeria enzymes turn the yellow broth grey or black. Staff read the result by eye, with no reader or luminometer.
Hygiena states the test detects fewer than 10 CFU of Listeria spp., and heat-injured L. monocytogenes at 10–50 CFU even with high levels of competing organisms. The large foam tip helps collect more of the sample.
Hygiena states InSite Listeria holds AOAC RI Performance Tested Methods℠ certification (#121902) for Listeria spp. on plastic, ceramic and stainless steel surfaces.
Steps are summarized from the Hygiena InSite Listeria instructions for use. Follow the full instructions.
| Result | What You See | Meaning |
|---|---|---|
| Negative | No colour change (yellow) | Listeria spp. not detected (final at 48 hours) |
| Presumptive positive | Grey or black on the swab or in the broth | Presumptive Listeria spp.; confirm |
A negative can only be recorded after 48 hours. Presumptive positives can be confirmed with PCR or a reference method such as Health Canada, FDA BAM, USDA MLG or ISO.
| Item | Notes |
|---|---|
| InSite Listeria devices | This product: 50 tests per box |
| Dry block incubator | 37 ± 1 °C (not included) |
| Sealing tape or film | Self-sealing tape, plastic wrap or paraffin film to seal the device (not included) |
| Product | InSite® Listeria |
| Item number | ILC050 |
| Pack | 50 tests per box |
| Detects | Listeria spp. (presumptive) |
| Sensitivity | <10 CFU Listeria spp. (per Hygiena) |
| Incubation | 37 ± 1 °C, 24–48 h |
| Result | Visual: yellow (negative), grey/black (presumptive positive) |
| Validation | AOAC RI PTM℠ #121902 (per Hygiena) |
| Storage | 2–8 °C |
| Manufacturer | Hygiena |
Specifications are from the Hygiena InSite Listeria instructions for use and product sheet.
Need the Safety Data Sheet or a certificate of analysis for a specific lot? Contact Cambridge and we'll request it from Hygiena.
Cambridge Environmental Products is a Canadian-owned laboratory supply distributor based in Ontario and has served Canadian labs since 1993. We supply Hygiena InSite environmental tests with CAD pricing and shipping across Canada. We work with food and beverage processors, QA/QC labs, sanitation managers and research laboratories, and can help with quotes and standing orders. Browse our lab supplies collection for more.
Listeria species on environmental surfaces. Results are presumptive and should be confirmed.
Presumptive positives can show from 24 hours. Negatives are recorded at 48 hours.
No. It detects Listeria spp. For a test that also flags L. monocytogenes, see Hygiena InSite L. mono Glo.
A dry block incubator at 37 °C and tape or film to seal the device.
Refrigerate at 2–8 °C and use by the expiry date.
Yes. Cambridge Environmental Products supplies Hygiena InSite Listeria across Canada with CAD pricing.
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