EC Medium, otherwise known as EC Broth, is used to detect the presence of coliform bacteria in drinking water, wastewater, and foods. Developed by Hajna and Perryl, it was designed for coliform bacteria, specifically E. coli. A buffered Lactose broth, it contains Bile Salts to inhibit Gram-positive bacteria and spore-forming microorganisms while enhancing that of E. coli. Coliforms can be detected by growth at 37°C, while Escherichia coli are detected by growth at 44.5°C. Dipotassium Phosphate and Monopotassium Phosphate act as buffering agents while Sodium Chloride maintains the osmotic balance of the medium. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 37 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute into test tubes containing inverted fermentation Durham tubes and autoclave at 121°C for 15 minutes.
E05-100 Product Information
E05-100 Safety Data Sheet