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Bile Esculin Azide Agar
Bile Esculin Azide Agar is used for isolating and presumptively identifying Group D Streptococci based on their ability to hydrolyze Esculin. This glucoside is hydrolyzed to esculetin and dextrose by the bacteria. The former reacts with Ferric Ammonium Citrate to form a dark brown or black complex. Oxgall inhibits Gram-positive bacteria not belonging to the Enterococci group, while Sodium Azide inhibits Gram-negative microorganisms. Beef Meat Extract, Pancreatic Digest of Casein, and Yeast Extract provide essential nitrogenous sources and carbohydrates for bacterial growth. Sodium Chloride maintains the osmotic balance and Agar is the gelifying agent. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 57 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.