Hygiena ZymoSnap ALP is a rapid test that measures alkaline phosphatase (ALP) in milk and dairy products to verify pasteurization. ALP is an enzyme naturally present in raw milk and destroyed by proper pasteurization, so a low ALP result confirms the product was heat-treated correctly. ZymoSnap uses bioluminescence and gives a quantitative result in about 5 minutes on the Hygiena EnSURE Touch or EnSURE luminometer.
Each device is an all-in-one test: add 75 µL of sample, snap to release the reagent, incubate for 5 minutes at 37 °C and read. Hygiena states the method is AOAC Performance Tested Method℠ validated, and that it measures below the 350 mU/L critical limit for ALP. It's sold in boxes of 100 single-use tests. Hygiena ZymoSnap ALP is available in Canada from Cambridge Environmental Products with CAD pricing and Canadian purchasing support.
The assay takes 5 minutes of incubation plus about 10 seconds to read. A problem with the pasteurizer can be caught and the product held before it leaves the plant.
Light output (RLU) is directly proportional to ALP activity. Hygiena states the test measures below the 350 mU/L critical limit for ALP in cow's milk, and compares its performance to the ISO 11816 / IDF 155 fluorometric reference method.
Hygiena states ZymoSnap ALP is validated under the AOAC Performance Tested Methods℠ program. The certificate is linked under Product Documents.
There are no reagents to prepare. Pipette the sample into the device, activate it and incubate. Results are stored on the luminometer and can be trended in Hygiena's SureTrend software. Hygiena states throughput is more than 45 tests per hour.
| Item | Notes |
|---|---|
| ZymoSnap ALP devices | This product: 100 tests per box |
| Hygiena luminometer | EnSURE Touch or EnSURE (not included) |
| 37 °C incubator | Dry block incubator or water bath at 37 ±1 °C (not included) |
| Pipette and tips | 75 µL (not included) |
| Positive control | ZymoSnap ALP Positive Control Kit (ZS-ALP-PC), sold separately, to set pass/fail limits |
Steps are from the Hygiena ZymoSnap ALP instructions for use. Pass/fail RLU limits must be set for each milk type using the positive control kit, and checked monthly and with each new lot.
| Application | How ZymoSnap ALP Helps |
|---|---|
| Batch release | Confirms pasteurization in minutes before product ships. |
| Pasteurizer checks | Catches under-processing or raw milk contamination quickly. |
| Multiple milk types | Separate pass/fail limits can be set for each fat level and product. |
| Records and trending | Results stored on the luminometer and in SureTrend software. |
| Product | ZymoSnap™ ALP Alkaline Phosphatase Test |
| Item number | ZS-ALP-100 (Hygiena store item ASY0347) |
| Pack | 100 tests per box |
| Method | Bioluminescence; quantitative ALP activity |
| Sample volume | 75 µL |
| Incubation | 5 min ±10 s at 37 ±1 °C |
| Read time | About 10 seconds |
| Critical limit | Measures below 350 mU/L ALP (per Hygiena) |
| Matrices | Cow's milk (whole, semi-skimmed, skimmed, flavoured), goat's milk, cream; Hygiena also lists yogurts and shakes |
| Not suitable for | Long-life or ambient-stable (e.g. UHT) milk products |
| Compatible instruments | Hygiena EnSURE Touch and EnSURE |
| Validation | AOAC Performance Tested Method℠ (per Hygiena) |
| Storage | 2–8 °C, in a cool dark place; keep in the bag until use (light sensitive) |
| Shelf life | Use by the expiry date on the label |
| Manufacturer | Hygiena |
Specifications are from the Hygiena ZymoSnap ALP instructions for use, product sheet and product page.
Cambridge Environmental Products is a Canadian-owned laboratory supply distributor based in Ontario and has served Canadian labs since 1993. We supply Hygiena ZymoSnap ALP tests with CAD pricing and shipping across Canada. We work with dairy processors, food and beverage QA/QC labs, contract testing labs and research laboratories, and can help with quotes and standing orders. Browse our lab supplies collection for more.
Alkaline phosphatase (ALP), an enzyme in raw milk that's destroyed by proper pasteurization. A low result confirms the product was pasteurized correctly.
About 5 minutes of incubation at 37 °C, plus about 10 seconds to read.
A Hygiena EnSURE Touch or EnSURE luminometer, a 37 °C incubator or water bath, a 75 µL pipette and the ZymoSnap Positive Control Kit.
Hygiena states ZymoSnap ALP is AOAC Performance Tested Method℠ validated. The certificate is linked above.
Yes. Hygiena lists cream, flavoured milks, yogurts and shakes, as well as cow's and goat's milk. It's not suitable for UHT or other ambient-stable products.
RLU readings depend on milk type and fat content. The positive control (350 mU/L) sets the pass/fail RLU for each product you test.
Yes. Cambridge Environmental Products supplies Hygiena ZymoSnap ALP across Canada with CAD pricing.
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