Hygiena InSite L. mono Glo is an all-in-one environmental swab test that screens for both Listeria species and Listeria monocytogenes in one device. After incubation at 37 °C, a grey or black colour shows presumptive Listeria spp. Then a green glow under a longwave UV lamp shows presumptive L. monocytogenes. Presumptive positives are visible from 24 hours and negatives are confirmed at 48 hours.
Hygiena states the test detects fewer than 10 CFU of Listeria spp. and L. monocytogenes, and holds AOAC Research Institute Performance Tested Methods℠ certification. It's sold in boxes of 50 tests. Hygiena InSite L. mono Glo is available in Canada from Cambridge Environmental Products with CAD pricing and Canadian purchasing support.
Colour change screens for Listeria spp., and UV fluorescence flags presumptive L. monocytogenes in the same device. You don't need a second test or a transfer step.
The pre-moistened foam swab, selective broth and indicators are in one sealed device. Hygiena states the broth includes a neutralizer to reduce sanitizer interference.
Hygiena states the test detects fewer than 10 CFU of Listeria spp. and of L. monocytogenes.
Hygiena states InSite L. mono Glo holds AOAC RI Performance Tested Methods℠ certification (#061802) for L. monocytogenes and six other Listeria species on plastic, ceramic and stainless steel surfaces. The medium can also be used as a primary enrichment for Hygiena BAX® System PCR.
Steps are summarized from the Hygiena InSite L. mono Glo instructions for use. Follow the full instructions.
| Result | What You See | Meaning |
|---|---|---|
| Negative | No colour change (amber/yellow) | Listeria spp. not detected (final at 48 hours) |
| Presumptive Listeria spp. | Grey or black, no green glow under UV | Presumptive Listeria spp.; confirm |
| Presumptive L. monocytogenes | Grey or black, with green glow under UV | Presumptive L. mono; confirm |
Don't apply UV if the broth stays amber or yellow. A negative can only be recorded after 48 hours. Confirm presumptives with PCR, ELISA, lateral flow or a reference method such as Health Canada, FDA BAM, USDA MLG or ISO.
| Item | Notes |
|---|---|
| InSite L. mono Glo devices | This product: 50 tests per box |
| Dry block incubator | 37 ± 1 °C (not included) |
| Longwave UV lamp | 360–400 nm, with UV safety glasses (not included) |
| Sealing tape or film | Self-sealing tape, plastic wrap or paraffin film (not included) |
| Product | InSite® L. mono Glo |
| Item number | ILMG050 |
| Pack | 50 tests per box |
| Detects | Listeria spp. and L. monocytogenes (presumptive) |
| Sensitivity | <10 CFU (per Hygiena) |
| Incubation | 37 ± 1 °C, 24–48 h |
| Result | Visual colour change (Listeria spp.); green fluorescence under 360–400 nm UV (L. mono) |
| Validation | AOAC RI PTM℠ #061802 (per Hygiena) |
| Storage | 2–8 °C |
| Manufacturer | Hygiena |
Specifications are from the Hygiena InSite L. mono Glo instructions for use and product sheet.
Need the Safety Data Sheet or a certificate of analysis for a specific lot? Contact Cambridge and we'll request it from Hygiena.
Cambridge Environmental Products is a Canadian-owned laboratory supply distributor based in Ontario and has served Canadian labs since 1993. We supply Hygiena InSite environmental tests with CAD pricing and shipping across Canada. We work with food and beverage processors, QA/QC labs, sanitation managers and research laboratories, and can help with quotes and standing orders. Browse our lab supplies collection for more.
Listeria species by colour change, and presumptive L. monocytogenes by green fluorescence under UV, from the same swab.
InSite Listeria detects Listeria spp. only. L. mono Glo adds a UV fluorescence check that flags presumptive L. monocytogenes.
Yes. A longwave UV lamp (360–400 nm) and UV safety glasses are needed to read the L. mono result. They're not included.
Presumptive positives can show from 24 hours. Negatives are recorded at 48 hours.
Refrigerate at 2–8 °C and use by the expiry date.
Yes. Cambridge Environmental Products supplies Hygiena InSite L. mono Glo across Canada with CAD pricing.
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