Eosin Methylene Blue Agar
Eosin Methylene Blue Agar
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Eosin Methylene Blue Agar is a solid medium used for the detection of Enterobacteriaceae. Pancreate Digest of Gelatin provides essential nutrients for bacterial growth. Sucrose and Lactose are the fermentable carbohydrates. Dipotassium Phosphate is the pH buffer. Agar is the gelifying agent. The dyes Eosin Y and Methylene Blue are toxic to most Gram-positive bacteria and serve as pH indicators. Rapid Lactose- or Sucrose-fermenting microorganisms produce sufficient quantities of acid to shift the color of the pH indicators to dark purple. E. coli produces a characteristic green metallic sheen. Non-fecal coliforms cannot ferment these carbohydrates and form normally-colored or colorless colonies on this medium. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 36 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121° C for 15 minutes.
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