Buffered Peptone Water
Buffered Peptone Water
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Buffered Peptone Water is used for pre-enriching damaged Salmonella spp. from various food sources. Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, high osmotic pressures, or pH changes can cause sub-lethal injury to Salmonella microorganisms. Pre-enrichment with Buffered Peptone Water allows for the repair of cell damage by maintaining a high pH over the pre-enrichment period due to the presence of Mono- and Disodium Phosphate. The high pH capacity is especially useful for vegetable samples, which have a low buffering capacity. There is a choice of 3 sizes - 500g, 2kg, and 10kg.
PREPARATION: Mix 20 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.
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