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Kikkoman LuciPac A3 Pen Aqua Swab System for TOTAL ATP+ADP+AMP
DescriptionLucipac Pen-AQUA was developed for the Lumitester PD-30 to examine water and other liquids for organic contamination in real time. Water and other liquids/waste water may contain ATP and/or AMP. These substances may be due to either insufficient cleaning or bacterial contamination. Using the Lucipac Pen-AQUA it is possible to assess the purity of liquids in just a few seconds. This allows, for example, an objective reading of the purity of "cleaning in place" water samples. The taking of liquids has been improved in the Lucipac Pen-AQUA; furthermore excellent reproducibility and accuracy are achieved. A much greater volume of liquid is examined than with a swab. Lucipac PenAQUA examines 150 μl of liquid, 50 % more than comparable products. The sample volume is taken with very high accuracy - deviations in sample volumes are less than 2 %.
Lumitester PD-30 with use of LuciPac®Pen allows rapid detection of food and microbial related residues on product contact surfaces as a monitoring tool for the effectiveness of implemented cleaning and disinfection. The quantitative detection of the intra- and extracellular Adenosine Triphosphates (ATP) as well as Adenosine Monophosphate (AMP) is carried out in the portable luminometer Lumitester PD-30. The instruments are not part of the test kit LuciPac®Pen .
The energy stored within the ATP molecule is released as light by splitting of ATP to AMP (Adenosine Monophosphate). The light can be measured quantitatively and corresponds with the ATP level. The reaction uses the enzyme luciferase from the well-known organism firefly:
Luciferin + Luciferase + ATP (Luciferin-Luciferase-AMP) + Pyrophosphate
(Luciferin-Luciferase-AMP) Oxyluciferin + Luciferase + CO2 + AMP+ Light
Via the integrated AMP-recycling system based on Pyruvate-Phosphate Dikinase (PPDK) AMP could be detected as well as ATP on the surfaces. Therefore the test is more sensitive as cleaning control then other ATP systems.