{"product_id":"hygiena-zymosnap-alp-positive-control-kit-350-mu-l-5-vials","title":"Hygiena ZymoSnap™ ALP Positive Control Kit – 350 mU\/L, 5 Vials","description":"\u003ch2\u003eHygiena ZymoSnap™ ALP Positive Control Kit – 350 mU\/L, 5 Vials\u003c\/h2\u003e\n\n\u003cp\u003eThe Hygiena ZymoSnap ALP Positive Control Kit contains 5 vials of alkaline phosphatase (ALP) standard at 350 mU\/L, the critical limit for pasteurization verification. You need it to set and check the pass\/fail limits for Hygiena ZymoSnap ALP tests. ZymoSnap results in RLU vary with milk type and fat content, so each product you test needs its own fail limit, set by running this control in that same milk.\u003c\/p\u003e\n\n\u003cp\u003eEach vial is reconstituted with a heat-treated negative control made from the milk you're testing, so the control matches your product. Hygiena lists expected ranges for 4%, 2% and 0.1% fat milk. The Hygiena ZymoSnap Positive Control Kit is available in Canada from Cambridge Environmental Products with CAD pricing and Canadian purchasing support.\u003c\/p\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003ch3 style=\"font-size:18px; line-height:1.35; margin-top:0;\"\u003eIdeal For\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSetting pass\/fail RLU limits\u003c\/strong\u003e for each milk type tested with ZymoSnap ALP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonthly verification\u003c\/strong\u003e of ZymoSnap ALP test performance\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eChecking each new lot\u003c\/strong\u003e of ZymoSnap ALP devices\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDairy QA\/QC labs\u003c\/strong\u003e documenting test and luminometer performance\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eWhy You Need It\u003c\/h2\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eSets your fail limit at 350 mU\/L\u003c\/h3\u003e\n\u003cp\u003eThe control contains ALP at 350 mU\/L. The average RLU of the positive control in a given milk becomes the fail limit for that product on your luminometer.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eMatched to your product\u003c\/h3\u003e\n\u003cp\u003eYou reconstitute each vial with a negative control made by heat-treating the same milk you test. The control then reflects the fat content and makeup of your own product.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eConfirms the system is working\u003c\/h3\u003e\n\u003cp\u003eRunning negative and positive controls when you start, monthly and with each new lot of devices confirms the tests, incubator and luminometer are all performing correctly.\u003c\/p\u003e\n\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003col style=\"margin:0;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMake the negative control:\u003c\/strong\u003e heat 10 mL of the milk you test at 72 °C for 10 minutes, cool quickly on ice, cover and refrigerate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTest the negative control:\u003c\/strong\u003e run 3 ZymoSnap ALP tests. The average must be below 20 RLU.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMake the positive control:\u003c\/strong\u003e remove the vial's seal and cap, add 1.0 mL of the matching negative control, recap and shake vigorously for 60 seconds until dissolved.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTest the positive control:\u003c\/strong\u003e run 3 ZymoSnap ALP tests and check the average against the expected range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSet the limit:\u003c\/strong\u003e enter the positive control average as the fail RLU for that milk type on your luminometer.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eSteps are from the Hygiena ZymoSnap ALP Positive Control Kit instructions. Use a separate vial and negative control for each milk type.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eExpected Positive Control Results\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; margin-top:12px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background:#f2f2f2;\"\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eMilk Fat\u003c\/th\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eMin RLU\u003c\/th\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eMax RLU\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e4%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e222\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e249\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e2%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e315\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e385\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e0.1%\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e548\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e784\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp\u003e\u003cem\u003eAverage of 3 replicates, per Hygiena. If the average falls outside the range, repeat 3 tests from the same vial.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\n\u003ctable style=\"width:100%; border-collapse:collapse; margin-top:12px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eZymoSnap™ ALP Positive Control Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eItem number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eZS-ALP-PC (Hygiena store item ASY0373)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eContents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e5 vials of ALP standard, 350 mU\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e1.0 mL heat-treated negative control (your own milk) per vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eStorage (unopened)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e2–8 °C; use by the expiry date on the box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eReconstituted control\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e2–8 °C, use within 7 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eNegative control\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eUse within 48 hours at 2–8 °C, or freeze at −20 °C for up to 3 weeks (single freeze-thaw)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eFor use with\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eHygiena ZymoSnap ALP tests (ZS-ALP-100) on the EnSURE Touch or EnSURE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eHygiena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cem\u003eSpecifications are from the Hygiena ZymoSnap ALP Positive Control Kit instructions and product page.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eSelection \u0026amp; Handling Notes\u003c\/h2\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#fff8e5;\"\u003e\n\u003cul style=\"margin:0;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eEquipment not included:\u003c\/strong\u003e you'll also need ZymoSnap ALP tests, a Hygiena EnSURE Touch or EnSURE, a 72 °C water bath or incubator, a 37 °C incubator, an ice bath, and pipettes for 75 µL and 1.0 mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOne milk type per vial.\u003c\/strong\u003e Reconstitute each vial only with negative control made from the milk type being tested.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTest the negative control first.\u003c\/strong\u003e It must average below 20 RLU before you prepare the positive control.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKeep samples chilled.\u003c\/strong\u003e Reactivated and microbial phosphatase can give high readings.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShort stability once mixed:\u003c\/strong\u003e use reconstituted controls within 7 days.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eProduct Documents\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hygiena.com\/documents\/63306\/zymosnap-alp-positive-control-kit-instructions-en.pdf\" target=\"_blank\" rel=\"noopener\"\u003eHygiena ZymoSnap ALP Positive Control Kit Instructions (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hygiena.com\/documents\/63301\/zymosnap-alp-instructions-en.pdf\" target=\"_blank\" rel=\"noopener\"\u003eHygiena ZymoSnap ALP Instructions for Use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNeed the Safety Data Sheet or a certificate of analysis for a specific lot? \u003ca href=\"\/pages\/contact\"\u003eContact Cambridge\u003c\/a\u003e and we'll request it from Hygiena.\u003c\/p\u003e\n\n\u003ch2\u003eCanadian Purchasing Support\u003c\/h2\u003e\n\u003cp\u003eCambridge Environmental Products is a Canadian-owned laboratory supply distributor based in Ontario and has served Canadian labs since 1993. We supply Hygiena ZymoSnap ALP positive control kits with CAD pricing and shipping across Canada. We work with dairy processors, food and beverage QA\/QC labs, contract testing labs and research laboratories, and can help with quotes and standing orders. Browse our \u003ca href=\"\/collections\/lab-supplies\"\u003elab supplies\u003c\/a\u003e collection for more.\u003c\/p\u003e\n\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eWhat is the positive control kit for?\u003c\/h3\u003e\n\u003cp\u003eIt sets and checks the pass\/fail RLU limits for ZymoSnap ALP tests. Each vial contains ALP at 350 mU\/L, the critical limit for pasteurization verification.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow many vials are in a kit?\u003c\/h3\u003e\n\u003cp\u003e5 vials. Each one is reconstituted with 1.0 mL of negative control made from one milk type.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow often should I run controls?\u003c\/h3\u003e\n\u003cp\u003eWhen you first set up, monthly, and with each new lot of ZymoSnap ALP devices, per Hygiena.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow long does a reconstituted vial last?\u003c\/h3\u003e\n\u003cp\u003eUp to 7 days at 2–8 °C.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eDo I need a separate vial for each milk type?\u003c\/h3\u003e\n\u003cp\u003eYes. Reconstitute each vial with negative control made from the same milk type you're setting the limit for.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eIs the kit available in Canada?\u003c\/h3\u003e\n\u003cp\u003eYes. Cambridge Environmental Products supplies the Hygiena ZymoSnap Positive Control Kit across Canada with CAD pricing.\u003c\/p\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003ch3 style=\"font-size:18px; line-height:1.35; margin-top:0;\"\u003eNeed Help Setting Up ALP Testing?\u003c\/h3\u003e\n\u003cp\u003eTell us:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe dairy products and fat levels you test\u003c\/li\u003e\n\u003cli\u003eWhich Hygiena luminometer you use\u003c\/li\u003e\n\u003cli\u003eHow many milk types you need limits for\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWe'll help you set up ZymoSnap testing and controls.\u003c\/p\u003e\n\u003ca href=\"\/pages\/contact\" style=\"display:inline-block; padding:12px 18px; background:#1f4e79; color:#ffffff; text-decoration:none; border-radius:5px; font-weight:bold;\"\u003e\nContact Cambridge for Product Support\n\u003c\/a\u003e\n\u003c\/div\u003e","brand":"Hygiena","offers":[{"title":"Default Title","offer_id":50052076437763,"sku":null,"price":337.03,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2006\/8997\/files\/ZymoSnap20Control_20Kit.webp?v=1793208011","url":"https:\/\/cambridgeenviro.com\/products\/hygiena-zymosnap-alp-positive-control-kit-350-mu-l-5-vials","provider":"Cambridge Environmental Products, Inc.","version":"1.0","type":"link"}