{"product_id":"hygiena-insite-l-mono-glo-swab-test","title":"Hygiena InSite® L. mono Glo – Listeria spp. \u0026 L. monocytogenes Swab Test, 50 Tests","description":"\u003ch2\u003eHygiena InSite® L. mono Glo – Listeria spp. \u0026amp; L. monocytogenes Swab Test, 50 Tests\u003c\/h2\u003e\n\n\u003cp\u003eHygiena InSite L. mono Glo is an all-in-one environmental swab test that screens for both \u003cem\u003eListeria\u003c\/em\u003e species and \u003cem\u003eListeria monocytogenes\u003c\/em\u003e in one device. After incubation at 37 °C, a \u003cstrong\u003egrey or black\u003c\/strong\u003e colour shows presumptive \u003cem\u003eListeria\u003c\/em\u003e spp. Then a \u003cstrong\u003egreen glow\u003c\/strong\u003e under a longwave UV lamp shows presumptive \u003cem\u003eL. monocytogenes\u003c\/em\u003e. Presumptive positives are visible from 24 hours and negatives are confirmed at 48 hours.\u003c\/p\u003e\n\n\u003cp\u003eHygiena states the test detects fewer than 10 CFU of \u003cem\u003eListeria\u003c\/em\u003e spp. and \u003cem\u003eL. monocytogenes\u003c\/em\u003e, and holds AOAC Research Institute Performance Tested Methods℠ certification. It's sold in boxes of 50 tests. Hygiena InSite L. mono Glo is available in Canada from Cambridge Environmental Products with CAD pricing and Canadian purchasing support.\u003c\/p\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003ch3 style=\"font-size:18px; line-height:1.35; margin-top:0;\"\u003eIdeal For\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-eat, dairy, meat and seafood\u003c\/strong\u003e plants where \u003cem\u003eL. monocytogenes\u003c\/em\u003e is the key concern\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnvironmental monitoring programs\u003c\/strong\u003e that want to separate \u003cem\u003eL. mono\u003c\/em\u003e from other \u003cem\u003eListeria\u003c\/em\u003e species\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePrioritizing investigations\u003c\/strong\u003e by flagging presumptive \u003cem\u003eL. mono\u003c\/em\u003e sites first\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIn-house screening\u003c\/strong\u003e before sending samples for confirmation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eWhy Choose InSite L. mono Glo?\u003c\/h2\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eTwo results from one swab\u003c\/h3\u003e\n\u003cp\u003eColour change screens for \u003cem\u003eListeria\u003c\/em\u003e spp., and UV fluorescence flags presumptive \u003cem\u003eL. monocytogenes\u003c\/em\u003e in the same device. You don't need a second test or a transfer step.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eAll-in-one device\u003c\/h3\u003e\n\u003cp\u003eThe pre-moistened foam swab, selective broth and indicators are in one sealed device. Hygiena states the broth includes a neutralizer to reduce sanitizer interference.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eSensitive screening\u003c\/h3\u003e\n\u003cp\u003eHygiena states the test detects fewer than 10 CFU of \u003cem\u003eListeria\u003c\/em\u003e spp. and of \u003cem\u003eL. monocytogenes\u003c\/em\u003e.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eAOAC validated\u003c\/h3\u003e\n\u003cp\u003eHygiena states InSite L. mono Glo holds AOAC RI Performance Tested Methods℠ certification (#061802) for \u003cem\u003eL. monocytogenes\u003c\/em\u003e and six other \u003cem\u003eListeria\u003c\/em\u003e species on plastic, ceramic and stainless steel surfaces. The medium can also be used as a primary enrichment for Hygiena BAX® System PCR.\u003c\/p\u003e\n\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003col style=\"margin:0;\"\u003e\n\u003cli\u003eSwab a 10 × 10 cm (4 × 4 in) area in a crisscross pattern, rotating the swab.\u003c\/li\u003e\n\u003cli\u003eReturn the swab to the tube, close the device and seal the joint with tape or film.\u003c\/li\u003e\n\u003cli\u003eBreak the SnapValve and squeeze the bulb 3–4 times to release the broth.\u003c\/li\u003e\n\u003cli\u003eMassage the bottom of the tube and shake for 3 seconds.\u003c\/li\u003e\n\u003cli\u003eIncubate at \u003cstrong\u003e37 ± 1 °C for 24–48 hours\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eIf the broth has turned grey or black, shine a \u003cstrong\u003elongwave UV lamp (360–400 nm)\u003c\/strong\u003e into the side of the tube bottom and look for green fluorescence. Wear UV safety glasses.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eSteps are summarized from the Hygiena InSite L. mono Glo instructions for use. Follow the full instructions.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eReading the Result\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; margin-top:12px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background:#f2f2f2;\"\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eResult\u003c\/th\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eWhat You See\u003c\/th\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eMeaning\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eNegative\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eNo colour change (amber\/yellow)\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e\n\u003cem\u003eListeria\u003c\/em\u003e spp. not detected (final at 48 hours)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003ePresumptive \u003cem\u003eListeria\u003c\/em\u003e spp.\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eGrey or black, no green glow under UV\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003ePresumptive \u003cem\u003eListeria\u003c\/em\u003e spp.; confirm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003ePresumptive \u003cem\u003eL. monocytogenes\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eGrey or black, with green glow under UV\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003ePresumptive \u003cem\u003eL. mono\u003c\/em\u003e; confirm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003cp\u003e\u003cem\u003eDon't apply UV if the broth stays amber or yellow. A negative can only be recorded after 48 hours. Confirm presumptives with PCR, ELISA, lateral flow or a reference method such as Health Canada, FDA BAM, USDA MLG or ISO.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eWhat You Need\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;\"\u003e\n\u003ctable style=\"width:100%; border-collapse:collapse; margin-top:12px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background:#f2f2f2;\"\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"border:1px solid #ddd; padding:10px; text-align:left;\"\u003eNotes\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eInSite L. mono Glo devices\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eThis product: 50 tests per box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eDry block incubator\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e37 ± 1 °C (not included)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eLongwave UV lamp\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e360–400 nm, with UV safety glasses (not included)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eSealing tape or film\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eSelf-sealing tape, plastic wrap or paraffin film (not included)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\n\u003ctable style=\"width:100%; border-collapse:collapse; margin-top:12px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eInSite® L. mono Glo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eItem number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eILMG050\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003ePack\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e50 tests per box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eDetects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e\n\u003cem\u003eListeria\u003c\/em\u003e spp. and \u003cem\u003eL. monocytogenes\u003c\/em\u003e (presumptive)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e\u0026lt;10 CFU (per Hygiena)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eIncubation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e37 ± 1 °C, 24–48 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eResult\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eVisual colour change (\u003cem\u003eListeria\u003c\/em\u003e spp.); green fluorescence under 360–400 nm UV (\u003cem\u003eL. mono\u003c\/em\u003e)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eValidation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eAOAC RI PTM℠ #061802 (per Hygiena)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003e2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px; width:40%;\"\u003e\u003cstrong\u003eManufacturer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"border:1px solid #ddd; padding:10px;\"\u003eHygiena\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cem\u003eSpecifications are from the Hygiena InSite L. mono Glo instructions for use and product sheet.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003ch2\u003eSelection \u0026amp; Handling Notes\u003c\/h2\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#fff8e5;\"\u003e\n\u003cul style=\"margin:0;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eUV lamp required.\u003c\/strong\u003e You need a longwave (360–400 nm) UV lamp and UV safety glasses to read the \u003cem\u003eL. mono\u003c\/em\u003e result. UV light is an eye and skin hazard.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResults are presumptive.\u003c\/strong\u003e Confirm positives with PCR or a reference method.\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eWait 48 hours for negatives.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eListeria-like organisms\u003c\/strong\u003e such as \u003cem\u003eEnterococcus\u003c\/em\u003e can also cause blackening. Expect more presumptives from floors and drains.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRefrigerate on receipt.\u003c\/strong\u003e Store at 2–8 °C and use by the expiry date.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTrained staff only.\u003c\/strong\u003e These tests can grow pathogens. Hygiena advises that pregnant women and immunocompromised people should not be near testing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDispose safely.\u003c\/strong\u003e Treat used devices as biohazards. Autoclave, incinerate, or soak unsealed devices in 20% bleach for 1 hour before disposal.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eProduct Documents\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hygiena.com\/documents\/63271\/insite-l-monocytogenes-glo-instructions-en.pdf\" target=\"_blank\" rel=\"noopener\"\u003eHygiena InSite L. mono Glo Instructions for Use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hygiena.com\/documents\/64306\/insite-l-monocytogenes-glo-product-sheet-en.pdf\" target=\"_blank\" rel=\"noopener\"\u003eHygiena InSite L. mono Glo Product Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNeed the Safety Data Sheet or a certificate of analysis for a specific lot? \u003ca href=\"\/fr\/pages\/contact\"\u003eContact Cambridge\u003c\/a\u003e and we'll request it from Hygiena.\u003c\/p\u003e\n\n\u003ch2\u003eCanadian Purchasing Support\u003c\/h2\u003e\n\u003cp\u003eCambridge Environmental Products is a Canadian-owned laboratory supply distributor based in Ontario and has served Canadian labs since 1993. We supply Hygiena InSite environmental tests with CAD pricing and shipping across Canada. We work with food and beverage processors, QA\/QC labs, sanitation managers and research laboratories, and can help with quotes and standing orders. Browse our \u003ca href=\"\/fr\/collections\/lab-supplies\"\u003elab supplies\u003c\/a\u003e collection for more.\u003c\/p\u003e\n\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eWhat does InSite L. mono Glo detect?\u003c\/h3\u003e\n\u003cp\u003e\u003cem\u003eListeria\u003c\/em\u003e species by colour change, and presumptive \u003cem\u003eL. monocytogenes\u003c\/em\u003e by green fluorescence under UV, from the same swab.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow is it different from InSite Listeria?\u003c\/h3\u003e\n\u003cp\u003eInSite Listeria detects \u003cem\u003eListeria\u003c\/em\u003e spp. only. L. mono Glo adds a UV fluorescence check that flags presumptive \u003cem\u003eL. monocytogenes\u003c\/em\u003e.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eDo I need a UV lamp?\u003c\/h3\u003e\n\u003cp\u003eYes. A longwave UV lamp (360–400 nm) and UV safety glasses are needed to read the \u003cem\u003eL. mono\u003c\/em\u003e result. They're not included.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow long does it take?\u003c\/h3\u003e\n\u003cp\u003ePresumptive positives can show from 24 hours. Negatives are recorded at 48 hours.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eHow should I store it?\u003c\/h3\u003e\n\u003cp\u003eRefrigerate at 2–8 °C and use by the expiry date.\u003c\/p\u003e\n\n\u003ch3 style=\"font-size:18px; line-height:1.35;\"\u003eIs InSite L. mono Glo available in Canada?\u003c\/h3\u003e\n\u003cp\u003eYes. Cambridge Environmental Products supplies Hygiena InSite L. mono Glo across Canada with CAD pricing.\u003c\/p\u003e\n\n\u003cdiv style=\"border:1px solid #d9d9d9; padding:18px; margin:24px 0; border-radius:8px; background:#f8f8f8;\"\u003e\n\u003ch3 style=\"font-size:18px; line-height:1.35; margin-top:0;\"\u003eNeed Help Setting Up Environmental Monitoring?\u003c\/h3\u003e\n\u003cp\u003eTell us:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWhich organisms your program monitors\u003c\/li\u003e\n\u003cli\u003eThe surfaces and zones you test\u003c\/li\u003e\n\u003cli\u003eWhether you have a 37 °C dry block incubator\u003c\/li\u003e\n\u003cli\u003eHow many swabs you run per week\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWe'll help you choose the right Hygiena tests and set up standing orders.\u003c\/p\u003e\n\u003ca href=\"\/fr\/pages\/contact\" style=\"display:inline-block; padding:12px 18px; background:#1f4e79; color:#ffffff; text-decoration:none; border-radius:5px; font-weight:bold;\"\u003e\nContact Cambridge for Product Support\n\u003c\/a\u003e\n\u003c\/div\u003e","brand":"Hygiena","offers":[{"title":"Default Title","offer_id":50053436342531,"sku":"ILMG050","price":705.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/2006\/8997\/files\/asy0433_insitel.monoglo_1_53adfbb2-67f0-44ae-a4a7-b23fa5b44d7a.png?v=1793214918","url":"https:\/\/cambridgeenviro.com\/fr\/products\/hygiena-insite-l-mono-glo-swab-test","provider":"Cambridge Environmental Products, Inc.","version":"1.0","type":"link"}